賀端華

Tuan-hua David Ho

研究室: Rm500

電話: 02-27899590 ext 108 傳真: 02-27827954

地址: 台北市南港區115研究院路二段128   

          中央研究院植物暨微生物學研究所

電子信箱: tho@sinica.edu.tw

學歷

博士(生化系密西根州立大學( 1976 )

學士(植物系臺灣大學( 1970 )

經歷與現職

1.  中央研究院植物研究所所長        2003 / 迄今

2.  華盛頓大學生物系教授兼副系主任   1998 / 迄今

3.  華盛頓大學生物系教授            1993 / 迄今

4.  華盛頓大學生物系副教授          19841992

5.  中央研究院分生所客座教授        1990 / 051990 / 12

6.  美國依立諾大學植物系助理教授    1978 / 081984 / 04

7. 麻省理工學院生物系博士後     1976 / 091978 / 08

研究興趣及專長

HORMONE AND STRESS-REGULATED GENE EXPRESSION IN PLANTS

We are interested in the mechanisms of developmental transition from embryogenesis to seed germination, a process governed by two phytohormones, gibberellins (GA) and abscisic acid (ABA). Since plant embryos are very resistant to environmental stresses such as drought and cold, we also study the role of stress-induced proteins in mediating stress tolerance in plants.

Hormonal control of gene expression in the aleurone cells of cereal grains

In this project we study the effect of GA and ABAon the expression of genes encoding (-amylases, proteases and nucleases in germinating cereal grains. We follow both biochemical and genetic approaches to investigate the structure and function of these genes and their protein products. We are currently investigating transcription factors, such as a Myb type DNA binding protein called GAMYB and a suppressor, HRT, which interact with specific regions in the (-amylase and protease gene promoters. We are also interested in elucidating the role of protein phosphatase 2C and a ser/thr protein kinase, PKABA1, on mediating the ABA-regulated gene expression. Future work includes the study of interactions among these factors and how hormones affect their levels and/or activities. The stress/ABA up-regulation of genes encoding late embryogenesis abundant (LEA) proteins has also been a subject of intensive study. We have determined that the promoter complex necessary and sufficient for ABAup-regulation consists of an ACGT box (Gbox) and a coupling element. We are currently cloning the transcription factors that specifically interact

  with these stress/ABA responsive promoter elements. We will study the effect of ABAtreatment on the sub-cellular distribution of these transcription factors. We also plan to use genetic mutants with altered hormone sensitivities to further elucidate the action of these hormones.

 

   Regulation and function of stress-induced genes

We are particularly interested in the function of drought, salinity and cold stress induced proteins in cereals and Arabidopsis. One of these proteins, HVA1, contains long stretches of amphipathic alpha-helical structure, and its over- expression in transgenic plants leads to elevated levels of stress tolerance. Another stress/ABA-induced protein, HVA22, has apparent homologs in many diverse eukaryotes, but not in any prokaryotes. Studies of the HVA22 homolog in yeast suggest that this protein be involved in vesicular transport that may be essential for modifications of the structure and function of plasma membrane. We are further investigating the function of these two stress/ABA induced protein following a multidisciplinary approach employing tools in cell biology, biochemistry and genetics. A patent on the use of HVA1 to generate stress tolerant plants has recently been granted. Efforts are underway to establish collaborations with industrial interests in further developing this technology.

Abscisic acid induced genes and drought resistance. The two tobacco plants shown are genetically identical except that the plant on the left has been engineered to overexpress a gene, HVA1, induced by abscisic acid or stress conditions. Overexpression of this gene allows the transgenic plant to withstand drought better than the control plant on the right, which is clearly wilted. Water was withheld from both plants on the same day and the moisture content of the soil is essentially identical for the two plants shown.